ClC-3 chloride channel protein induces G1�arrest in hepatocellular carcinoma Hep3B cells
نویسندگان
چکیده
منابع مشابه
Downregulation of Kinesin Spindle Protein Inhibits Proliferation, Induces Apoptosis and Increases Chemosensitivity in Hepatocellular Carcinoma Cells
Background: Kinesin spindle protein (KSP) plays a critical role in mitosis. Inhibition of KSP function leads to cell cycle arrest at mitosis and ultimately to cell death. The aim of this study was to suppress KSP expression by specific small-interfering RNA (siRNA) in Hep3B cells and evaluate its anti-tumor activity. Methods: Three siRNA targeting KSP (KSP-siRNA #1-3) and one mismatched-siRNA (...
متن کاملdownregulation of kinesin spindle protein inhibits proliferation, induces apoptosis and increases chemosensitivity in hepatocellular carcinoma cells
background: kinesin spindle protein (ksp) plays a critical role in mitosis. inhibition of ksp function leads to cell cycle arrest at mitosis and ultimately to cell death. the aim of this study was to suppress ksp expression by specific small-interfering rna (sirna) in hep3b cells and evaluate its anti-tumor activity. methods: three sirna targeting ksp (ksp-sirna #1-3) and one mismatched-sirna (...
متن کاملThe swelling-activated chloride channel ClC-2, the chloride channel ClC-3, and ClC-5, a chloride channel mutated in kidney stone disease, are expressed in distinct subpopulations of renal epithelial cells.
The mammalian genome encodes at least nine different members of the ClC family of chloride channels. So far only two of them could be localized on a cellular level in the kidney. We now report on the precise intrarenal localization of the mRNAs coding for the chloride channels ClC-2, ClC-3 and ClC-5. Expression of ClC-2 mRNA, encoding a swelling-activated chloride channel, could be demonstrated...
متن کاملClC-3 is a candidate of the channel proteins mediating acid-activated chloride currents in nasopharyngeal carcinoma cells.
Acid-activated chloride currents have been reported in several cell types and may play important roles in regulation of cell function. However, the molecular identities of the channels that mediate the currents are not defined. In this study, activation of the acid-induced chloride current and the possible candidates of the acid-activated chloride channel were investigated in human nasopharynge...
متن کاملThe ClC-3 chloride channel promotes acidification of lysosomes in CHO-K1 and Huh-7 cells.
ClC-3 is a voltage-gated Cl- channel that is highly conserved and widely expressed, although its function, localization, and properties remain a matter of considerable debate. In this study, we have shown that heterologous expression of ClC-3 in either Chinese hamster ovary (CHO-K1) or human hepatoma (Huh-7) cells results in the formation of large, acidic vesicular structures within cells. Vesi...
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ژورنال
عنوان ژورنال: Oncology Reports
سال: 2018
ISSN: 1021-335X,1791-2431
DOI: 10.3892/or.2018.6416